Circaflux

Circaflux

2013

NUMBER OF ROOMS USED

PROJECT DURATION

3 MONTHS

GOALS

To investigate the impact of the circadian rhythm of two plant species (beans and cotton) on the regulation of their stomatal conductance and on their gas exchange.

A new study on circadian rhythms

Consequences

This study builds on previous work (Resco de Dios et al., Ecology Letters, (2009) 12: 583–592; Resco de Dios et al., Global Change Biology, (2012) 18, 1956–1970; Resco de Dios et al., New Phytologist, in press), to understand the role of the circadian clock in ecosystem processes. This previous work provided circumstantial evidence of the circadian regulation of net CO₂ exchange and water by the ecosystem, as well as BVOC emissions, by combining filtration and modelling techniques to minimise environmental variation in the datasets and short (12-hour) overnight sampling intervals. However, we still lack a direct and unequivocal test of the circadian regulation of gas exchange beyond the leaf level and in the field. The only direct test of the circadian regulation of NEE, ET and BVOC emissions at the ecosystem level is continuous monitoring over a period of ~24/48 hours under constant levels of light, temperature and other environmental factors (hereinafter referred to as the ‘continuous environment’).

Treatments

Two crops, cotton and beans, studied in succession; two experimental phases: no water restriction, drought. Each phase lasts two weeks for one crop. The choice of the first crop will depend on the development of the canopies. When the second crop begins to be studied, the first is subjected to drought conditions. This is followed by four weeks of study under good water conditions and then four weeks under drought conditions.

Each phase consists of two sub-phases : one for studying the circadian rhythm under constant light and another for studying the circadian rhythm under constant darkness. The study of the circadian rhythm involves comparing physiology under variable daytime conditions with that under constant conditions. The variable conditions are controlled (created using artificial lighting to ensure similar conditions regardless of the weather outside and to ensure that the maximum light level is equal to the constant light level provided by the lamps).

Each phase lasts two weeks and involves the following sequence of activities each day.

Researchers

Arthur Gessler ; Jacques Roy ; Zachary Kayler ; Juan Pedro Ferrio ; Josu Alday ; Michael Bahn ; Jorge del Castillo ; Víctor Resco de Dios ; Sébastien Devidal ; Sonia García-Muñoz ; Damien Landais ; Paula Martín-Gómez ; Alexandru Milcu ; Clément Piel ; Karin Pirhofer-Walzl ; Serajis Salekin ; David Tissue ; Mark Tjoelker ; Jordi Voltas ; William RL Anderegg

Publications

de Dios, V. R., Roy, J., Ferrio, J. P., Alday, J. G., Landais, D., Milcu, A., & Gessler, A. (2015). Processes driving nocturnal transpiration and implications for estimating land evapotranspiration. Scientific Reports, 5, 10975.

See

Resco de Dios, V., Gessler, A., … Roy, J. (2016). Circadian rhythms have significant effects on leaf-to-canopy scale gas exchange under field conditions. GigaScience, 5(1), 43.

See

Gessler, A., Roy, J., … de Dios, V. R. (2017). Night and day – Circadian regulation of night-time dark respiration and light-enhanced dark respiration in plant leaves and canopies. Environmental and Experimental Botany, 137, 14–25.

See

García-Plazaola, J. I., Fernández-Marín, … Resco de Dios, V. (2017). Endogenous circadian rhythms in pigment composition induce changes in photochemical efficiency in plant canopies. Plant Cell and Environment, 40(7), 1153–1162.

See

Resco de Dios, V., Anderegg, W. R. L., … Gessler, A. (2020). Circadian Regulation Does Not Optimize Stomatal Behaviour. Plants, 9, 1091.

See

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